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Beta-Catenin兔单抗
人阅读 发布时间:2022-06-27 09:01
来源 | 用途 | 交叉反应性 | 分子量 |
Rabbit | WB, IP, IF, IHC, ICC | H, M, R | 92.0kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
1:500-1:5,000 | 1:10-1:50 | 1:20-1:50 | 1:20-1:200 | 1:20-1:50 | - | - | - |
抗体详细信息如下:
About this Antibody | |
Name | Beta-Catenin Rabbit Monoclonal Antibody |
Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
Isotype | IgG |
Purification | Affinity purification |
About the Immunogen | |
Immunogen | Recombinant protein |
Gene ID | 1499(Human); 12387(Mouse); 84353(Rat) |
SwissProt | P35222(Human); Q02248(Mouse); Q9WU82(Rat) |
Synonyms | CTNNB; MRD19; armadillo |
Category | Stem Cells & Wnt; Neuronal Signaling; Cytoskeletal Signaling |
Background | β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. |